Much of our understanding of the structural organisation of the living cell has come about through recent advances in fluorescence-labelling of target molecules and laser scanning
microscopy. With the release of DirectFRAP from Carl Zeiss, scientists can now make similar strides in probing the dynamics of membrane transport and the movement of molecules
within the living cell. FRAP, FLIP, photoactivation, conversion of Dendra, on-off switching of Dronpa and other photomanipulation techniques, use a combination of intense pulses of laser light and widefield epi-fluorescence observation to measure the movement of fluorescent markers within the cell. Fitted to the Carl
Zeiss Axio Observer microscope, DirectFRAP overcomes the dynamic compromises inherent in previous systems by eliminating the link between laser intensity and the size of the ROI.
Polls
Loading ...