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 Applied Biosystems has launched a new family of TaqMan Cells-to-CT Kits for performing cell-based gene expression research more rapidly. The kits eliminate the time-consuming step of RNA purification, reducing the amount of time that is typically necessary to prepare and analyse RNA from cultured cells using real-time PCR in applications such as siRNA-mediated
gene silencing and microRNA analysis. The TaqMan Cells-to-CT Kits allow expression analysis to be performed directly from cultured mammalian cells derived from a variety of tissue sources, including epithelial cells and stem cells. Sample preparation time is reduced to under ten minutes, compared to the traditionally required 30 to 60 minutes, and loss of RNA is eliminated.
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